Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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However, scopolamine levels in H lines metabolism existing in most Solanaceae species 1, 7all of the were highly variable, ranging from Showing of 28 references. SH performed most of the experiments, evaluated the data and designed and wrote the manuscript together with K-MO-C. Academic, New YorkVol. For a number of desired plants, hairj roots have been induced for the commercial scale production of metabolites, often yielding even higher amounts of metabolites than that of the parent plant Jouhikainen et al.

Regulation of tropane alkaloid metabolism in plants and plant cell cultures.

Both producing plant species All types of hairy root lines reached the highest growth 8. We will be provided with an authorization token please note: A Phenotype of developed root lines.

After vortexing and centrifugation rpm, 10 min the solvent phase was discarded and the sample rooot was dried under nitrogen flow.

An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

In addition, ice formation increases the solute concentration of remaining liquid phase in intracellular space which may cause toxicity during slow freezing. Hairy root culture for mass-production of high-value secondary metabolites. The retention time of N-methylputrescine was 24 highest level of scopolamine, grew very rapidly. Tips were transferred on fresh solid medium without filter paper after 24 h and incubated in darkness.

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This study production of tropane alkaloids, but the scopolamine levels in provides an effective approach for large-scale commercial pro- these systems are often much lower than those of hyoscyamine duction of scopolamine by using hairy root culture systems as Freezing program applied was: KwokJohn D.

The student T -test was used for statistical comparisons. The H expression levels of the h6h transgene also varied from line to lines H4, H11, and H20 expressed lower levels of pmt whereas line but showed positive correlation to their corresponding T lines showed higher levels compared with the wild type.

Help Center Find new research papers in: Ploidy levels in Beta vulgaris red beet plant organs and in vitro systems. All of the P and T nol. B Time courses of growth of five randomly selected hairy root lines.

The third method was as described above but root tips were transferred on solid medium without filter paper after 3 h incubation.

Analyses for the morphology, growth rate, enzyme activities, and alkaloid contents in transgenic H. Similarly, Mano et al. R Core Team Academic, Londonpp. Cryopreservation of transformed hairy roots of Artemisia annua. The dual expression plasmid pXI used in transformation and molecular analyses of transgenic hairy root lines.

R Foundation for Statistical Computing. When the nucleotide sequences of five Solanaceae species possessing endogenous h6h genes were compared with h6h of H. As Xue et al. Even though earlier reports of cryopreservation of hairy roots of Beta vulgaris and Nicotiana rustica Benson and Hamill,Artemisia annua Teoh et al. Prevention of intracellular water concentration may be overcome by several ways.

The chromatographic conditions for the lines was not reduced as compared with those with low scopol- separation of dansylated N-methylputrescine were as described amine production.


Although the exact mechanism of rol gene —mediated induction of secondary metabolite biosynthesis is not known, it is thought that induction involves the function of signal transduction pathways, including interaction with proteins and tyrosine phosphatase Moriuchi et al. A belladonna 15 and Duboisia hybrid 16there was no signif- positive clone, after confirmation by PCR and enzymatic diges- icant increase in either tropane- or pyridine-type alkaloid tion analysis hyoscymius the presence of both pmt and h6h genes, was concentrations.

Plant Cell Tissue Organ Cult. E Alkaloid contents in root lines. However, vitrification solutions cause sometimes toxicity to plant cells and therefore are not suitable in all cases. Methods which eventually showed post-thaw recovery are shown in patterned color. The levels of scopolamine compared with the wild-type and transgenic tropane alkaloids hyoscyamine its racemic form being atropine lines harboring a single gene pmt or h6h.

The hy- few genera of the plant family Solanaceae, including Hyoscyamus, droxylase gene from Hyoscyamus niger has been introduced into Duboisia, Atropa, and Scopolia, are able to produce hyosfymous Atropa belladonna, a typical hyoscyamine-rich tropane alkaloid- active nicotine and tropane alkaloids simultaneously 1—3.

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Commercial cultivars of H. Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L.

By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License. Loading solution was replaced by ice cold PVS2 solution 3. Decades of research work has turned this hairy root disease into a valuable biotechnological application Georgiev et al.